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To generate cDNA (complementary DNA) Gentaur uses these steps:  Isolation of RNA: Extract RNA from the desired tissue or cell samples. This can be done using commercially available RNA isolation kits, such as TRIzol or RNeasy kits. Ensure that the RNA isolation method chosen is appropriate for the specific sample type.Reverse Transcription (RT): Perform reverse transcription to convert the isolated RNA into cDNA. This involves using reverse transcriptase enzyme to synthesize cDNA …

Western blotting is an important technique used in cell and molecular biology. By using a western blot, researchers are able to identify specific proteins from a complex mixture of proteins extracted from cells. The technique uses three elements to accomplish this task: (1) separation by size, (2) transfer to a solid support, and (3) marking target protein using a proper primary and secondary antibody to visualize. This paper will attempt to explain the technique and theory behind western b …

Monoclonal and Polycolnals Antibodies

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